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Supplementary Figure legendsSupplementary Figure 2. Phenotypic and functional characterization ofPD-MSC. (A) Morphology and karyotype (44+XX) of PD-MSC. PD-MSC showed…
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Supplementary Figure legendsSupplementary Figure 2. Phenotypic and functional characterization ofPD-MSC. (A) Morphology and karyotype (44+XX) of PD-MSC. PD-MSC showed fibroblastic-like morphology. Magnification:x100 (B) Cytometric analysis of PD-MSC for the MSC marker CD44, CD73, CD90 and CD105, the hematopoeitic/endothelial cell marker CD34, CD31, CD45 and the MSC migration marker CD29, CD49 and the immune suppression marker CD54, CD102, CD106, CD166 and non-trophoblast marker CD9. (C) PD-MSC single cell size is average 14.3μm. (D) Population doubling level in PD-MSC. (E) ELISA assay of pro-angiogenic factor/neurotropic factor (VEGF, TGFß1) released by PD-MSC. (F) Differentiation potential of PD-MSC. Magnification:x100BACD73CD105CD44CD90CD31CD29CD34CD45Cell countsCD54CD166CD49CD106CDSSEA4HLA-DRCD9HLA-ABCFluorescenceEFOsteocyte(Von kossa)Chondrocyte(Alcian blue)Adipocyte(Oil red O)CONPD-MSCCONPD-MSC
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