basics of microscopy and staining

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this is a series of lectures on microbiology useful for undergraduates and paramedical students
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  Fundamental Techniques in Microbiology Microscopy and Staining Dr. Ashish V. Jawarkar (M.D.)Parul Sevashram Hospital  Fundamental Techniques MicroscopyStaining Aseptic techniqueSterilization and waste disposal  Microscopy Measurement  Microorganisms are very small  Use metric system  Metre (m) : standard unit  Micrometre (  m) = 1 x10 -6 m  Nanometre (nm) = 1 x10 -9 m   Angstrom (Å) = 1 x10 -10 m  Terms Relevant to Microscopy Total Magnification  Eyepiece x objective lens Resolution   Ability of the lens to distinguish two points asseparate  Theoretical limit for light microscope is 0.2  m
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